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avidin biotin immunoperoxidase complex abc  (Vector Laboratories)


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    Vector Laboratories avidin biotin immunoperoxidase complex abc
    Immunohistochemical observations of MCP-1 protein in the spinal cord of SJL and G1H+/− mice sacrificed at presymptomatic (9 w) and postsymptomatic (15 w) stages (n = 3 in each group). Inset indicates a vacuolated neuron. Immunoreaction product deposits are visualized by the <t>avidin-biotin-immunoperoxidase</t> complex method using 3,3’-diaminomenzidine tetrahydrochloride and hematoxylin as the chromogen and counterstain, respectively, by light microscopy. Scale bars indicate 100 μm (panels) and 50 μm (inset).
    Avidin Biotin Immunoperoxidase Complex Abc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 9352 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin immunoperoxidase complex abc/product/Vector Laboratories
    Average 96 stars, based on 9352 article reviews
    avidin biotin immunoperoxidase complex abc - by Bioz Stars, 2026-02
    96/100 stars

    Images

    1) Product Images from "MCP-1/CCR2 signaling-mediated astrocytosis is accelerated in a transgenic mouse model of SOD1-mutated familial ALS"

    Article Title: MCP-1/CCR2 signaling-mediated astrocytosis is accelerated in a transgenic mouse model of SOD1-mutated familial ALS

    Journal: Acta Neuropathologica Communications

    doi: 10.1186/2051-5960-1-21

    Immunohistochemical observations of MCP-1 protein in the spinal cord of SJL and G1H+/− mice sacrificed at presymptomatic (9 w) and postsymptomatic (15 w) stages (n = 3 in each group). Inset indicates a vacuolated neuron. Immunoreaction product deposits are visualized by the avidin-biotin-immunoperoxidase complex method using 3,3’-diaminomenzidine tetrahydrochloride and hematoxylin as the chromogen and counterstain, respectively, by light microscopy. Scale bars indicate 100 μm (panels) and 50 μm (inset).
    Figure Legend Snippet: Immunohistochemical observations of MCP-1 protein in the spinal cord of SJL and G1H+/− mice sacrificed at presymptomatic (9 w) and postsymptomatic (15 w) stages (n = 3 in each group). Inset indicates a vacuolated neuron. Immunoreaction product deposits are visualized by the avidin-biotin-immunoperoxidase complex method using 3,3’-diaminomenzidine tetrahydrochloride and hematoxylin as the chromogen and counterstain, respectively, by light microscopy. Scale bars indicate 100 μm (panels) and 50 μm (inset).

    Techniques Used: Immunohistochemical staining, Avidin-Biotin Assay, Light Microscopy

    Immunohistochemical (a), immunoblot (b) and densitometric (c) analyses for CCR2 protein in the spinal cord of SJL and G1H+/− mice sacrificed at presymptomatic (9 w), onset (12 w) and postsymptopatic (15 w) stages. Immunoreaction product deposits are visualized by the avidin-biotin-immunoperoxidase complex method using 3,3’-diaminomenzidine tetrahydrochloride and hematoxylin as the chromogen and counterstain, respectively, by light microscopy. Scale bar indicates 100 μm ( a ). Electrophoretic mobility ( b ) and optical density ( c ) are compared between the postsymptomatic SJL and G1H+/− groups (n = 5 in each group). Two-way ANOVA provides P < 0.05. Posthoc Bonferroni correction provides *P < 0.05 as compared to the SJL group.
    Figure Legend Snippet: Immunohistochemical (a), immunoblot (b) and densitometric (c) analyses for CCR2 protein in the spinal cord of SJL and G1H+/− mice sacrificed at presymptomatic (9 w), onset (12 w) and postsymptopatic (15 w) stages. Immunoreaction product deposits are visualized by the avidin-biotin-immunoperoxidase complex method using 3,3’-diaminomenzidine tetrahydrochloride and hematoxylin as the chromogen and counterstain, respectively, by light microscopy. Scale bar indicates 100 μm ( a ). Electrophoretic mobility ( b ) and optical density ( c ) are compared between the postsymptomatic SJL and G1H+/− groups (n = 5 in each group). Two-way ANOVA provides P < 0.05. Posthoc Bonferroni correction provides *P < 0.05 as compared to the SJL group.

    Techniques Used: Immunohistochemical staining, Western Blot, Avidin-Biotin Assay, Light Microscopy



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    Immunohistochemical observations of MCP-1 protein in the spinal cord of SJL and G1H+/− mice sacrificed at presymptomatic (9 w) and postsymptomatic (15 w) stages (n = 3 in each group). Inset indicates a vacuolated neuron. Immunoreaction product deposits are visualized by the <t>avidin-biotin-immunoperoxidase</t> complex method using 3,3’-diaminomenzidine tetrahydrochloride and hematoxylin as the chromogen and counterstain, respectively, by light microscopy. Scale bars indicate 100 μm (panels) and 50 μm (inset).
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    Immunohistochemical observations of MCP-1 protein in the spinal cord of SJL and G1H+/− mice sacrificed at presymptomatic (9 w) and postsymptomatic (15 w) stages (n = 3 in each group). Inset indicates a vacuolated neuron. Immunoreaction product deposits are visualized by the <t>avidin-biotin-immunoperoxidase</t> complex method using 3,3’-diaminomenzidine tetrahydrochloride and hematoxylin as the chromogen and counterstain, respectively, by light microscopy. Scale bars indicate 100 μm (panels) and 50 μm (inset).
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    Immunohistochemical observations of MCP-1 protein in the spinal cord of SJL and G1H+/− mice sacrificed at presymptomatic (9 w) and postsymptomatic (15 w) stages (n = 3 in each group). Inset indicates a vacuolated neuron. Immunoreaction product deposits are visualized by the <t>avidin-biotin-immunoperoxidase</t> complex method using 3,3’-diaminomenzidine tetrahydrochloride and hematoxylin as the chromogen and counterstain, respectively, by light microscopy. Scale bars indicate 100 μm (panels) and 50 μm (inset).
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    Immunohistochemical observations of MCP-1 protein in the spinal cord of SJL and G1H+/− mice sacrificed at presymptomatic (9 w) and postsymptomatic (15 w) stages (n = 3 in each group). Inset indicates a vacuolated neuron. Immunoreaction product deposits are visualized by the <t>avidin-biotin-immunoperoxidase</t> complex method using 3,3’-diaminomenzidine tetrahydrochloride and hematoxylin as the chromogen and counterstain, respectively, by light microscopy. Scale bars indicate 100 μm (panels) and 50 μm (inset).
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    Examples of optimal and suboptimal concordance with PD-L1 IHC reference stains for different protocols during the 2018 EQA scheme. Images represent a matching core with a validated consensus TPS of > 50%. The core was part of a tissue micro-array containing four different FFPE cases for staining by routine antibodies and detection systems of the 2018 EQA participants. Protocols are presented as reported by the participating laboratories. Scale bar 1 mm. Optimal concordance (top row, from left to right): SP263: RTU antibody from Ventana (16 min incubation at 36 °C) in combination with the Ventana CC1 (64 min.) and OptiView DAB IHC Detection Kit. 22C3: Antibody from Dako (diluted 1/40, 16-min incubation at 37 °C) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit. 28-8: Antibody from Abcam (diluted 1/100, 32-min incubation at RT) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit. SP142: RTU antibody from Ventana (24-min incubation at 37 °C) in combination with the Ventana CC1 (48 min) and UltraView DAB IHC Detection Kit. E1L3N: Antibody from Cell Signaling (diluted 1/200, 30-min incubation at RT) in combination with Leica Bond Epitope Retrieval 2 (20 min) and bond polymer refine detection system. Suboptimal concordance (bottom row, from left to right): SP263: RTU antibody from Ventana (60-min incubation at 37 °C) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit; weak demonstration of antigen in the tumor population and cytoplasmic staining. 22C3: Antibody from Dako (diluted 1/50, 30-min incubation at RT) in combination with Dako EnVisionFLEX Target Retrieval Solution (low pH) and the Envision Flex detection system; Excessive background staining and cytoplasmic staining. 28-8: Antibody from Abcam (diluted 1/50, 20-min incubation at 32 °C) in combination with Dako EnVisionFLEX Target Retrieval Solution (low pH) and the Envision Flex detection system; background staining. SP142: RTU antibody from Ventana (16-min incubation at 36 °C) in combination with the Ventana CC1 (48 min) and OptiView DAB IHC Detection Kit; weak staining of epithelial cells. E1L3N: Antibody from Cell Signaling (diluted 1/150, 60-min incubation at RT) in combination with laboratory developed antigen retrieval by TRIS-EDTA and the Vectastain <t>ABC</t> <t>immunoperoxidase</t> staining avidin-biotin complexes; weak demonstration of antigen in the tumor population and cytoplasmic staining. Abbreviations: EQA external quality assessment, FFPE formalin-fixed paraffin embedded, IHC immunohistochemistry, PD-L1 programmed death ligand 1, RT room temperature, RTU ready-to-use, TPS tumor proportion score
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    Image Search Results


    Immunohistochemical observations of MCP-1 protein in the spinal cord of SJL and G1H+/− mice sacrificed at presymptomatic (9 w) and postsymptomatic (15 w) stages (n = 3 in each group). Inset indicates a vacuolated neuron. Immunoreaction product deposits are visualized by the avidin-biotin-immunoperoxidase complex method using 3,3’-diaminomenzidine tetrahydrochloride and hematoxylin as the chromogen and counterstain, respectively, by light microscopy. Scale bars indicate 100 μm (panels) and 50 μm (inset).

    Journal: Acta Neuropathologica Communications

    Article Title: MCP-1/CCR2 signaling-mediated astrocytosis is accelerated in a transgenic mouse model of SOD1-mutated familial ALS

    doi: 10.1186/2051-5960-1-21

    Figure Lengend Snippet: Immunohistochemical observations of MCP-1 protein in the spinal cord of SJL and G1H+/− mice sacrificed at presymptomatic (9 w) and postsymptomatic (15 w) stages (n = 3 in each group). Inset indicates a vacuolated neuron. Immunoreaction product deposits are visualized by the avidin-biotin-immunoperoxidase complex method using 3,3’-diaminomenzidine tetrahydrochloride and hematoxylin as the chromogen and counterstain, respectively, by light microscopy. Scale bars indicate 100 μm (panels) and 50 μm (inset).

    Article Snippet: Antibody binding was visualized by the avidin-biotin-immunoperoxidase complex (ABC) method using the appropriate Vectastain ABC kit (Vector Laboratories, Burlingame, CA, USA) according to the manufacturer’s instructions.

    Techniques: Immunohistochemical staining, Avidin-Biotin Assay, Light Microscopy

    Immunohistochemical (a), immunoblot (b) and densitometric (c) analyses for CCR2 protein in the spinal cord of SJL and G1H+/− mice sacrificed at presymptomatic (9 w), onset (12 w) and postsymptopatic (15 w) stages. Immunoreaction product deposits are visualized by the avidin-biotin-immunoperoxidase complex method using 3,3’-diaminomenzidine tetrahydrochloride and hematoxylin as the chromogen and counterstain, respectively, by light microscopy. Scale bar indicates 100 μm ( a ). Electrophoretic mobility ( b ) and optical density ( c ) are compared between the postsymptomatic SJL and G1H+/− groups (n = 5 in each group). Two-way ANOVA provides P < 0.05. Posthoc Bonferroni correction provides *P < 0.05 as compared to the SJL group.

    Journal: Acta Neuropathologica Communications

    Article Title: MCP-1/CCR2 signaling-mediated astrocytosis is accelerated in a transgenic mouse model of SOD1-mutated familial ALS

    doi: 10.1186/2051-5960-1-21

    Figure Lengend Snippet: Immunohistochemical (a), immunoblot (b) and densitometric (c) analyses for CCR2 protein in the spinal cord of SJL and G1H+/− mice sacrificed at presymptomatic (9 w), onset (12 w) and postsymptopatic (15 w) stages. Immunoreaction product deposits are visualized by the avidin-biotin-immunoperoxidase complex method using 3,3’-diaminomenzidine tetrahydrochloride and hematoxylin as the chromogen and counterstain, respectively, by light microscopy. Scale bar indicates 100 μm ( a ). Electrophoretic mobility ( b ) and optical density ( c ) are compared between the postsymptomatic SJL and G1H+/− groups (n = 5 in each group). Two-way ANOVA provides P < 0.05. Posthoc Bonferroni correction provides *P < 0.05 as compared to the SJL group.

    Article Snippet: Antibody binding was visualized by the avidin-biotin-immunoperoxidase complex (ABC) method using the appropriate Vectastain ABC kit (Vector Laboratories, Burlingame, CA, USA) according to the manufacturer’s instructions.

    Techniques: Immunohistochemical staining, Western Blot, Avidin-Biotin Assay, Light Microscopy

    Examples of optimal and suboptimal concordance with PD-L1 IHC reference stains for different protocols during the 2018 EQA scheme. Images represent a matching core with a validated consensus TPS of > 50%. The core was part of a tissue micro-array containing four different FFPE cases for staining by routine antibodies and detection systems of the 2018 EQA participants. Protocols are presented as reported by the participating laboratories. Scale bar 1 mm. Optimal concordance (top row, from left to right): SP263: RTU antibody from Ventana (16 min incubation at 36 °C) in combination with the Ventana CC1 (64 min.) and OptiView DAB IHC Detection Kit. 22C3: Antibody from Dako (diluted 1/40, 16-min incubation at 37 °C) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit. 28-8: Antibody from Abcam (diluted 1/100, 32-min incubation at RT) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit. SP142: RTU antibody from Ventana (24-min incubation at 37 °C) in combination with the Ventana CC1 (48 min) and UltraView DAB IHC Detection Kit. E1L3N: Antibody from Cell Signaling (diluted 1/200, 30-min incubation at RT) in combination with Leica Bond Epitope Retrieval 2 (20 min) and bond polymer refine detection system. Suboptimal concordance (bottom row, from left to right): SP263: RTU antibody from Ventana (60-min incubation at 37 °C) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit; weak demonstration of antigen in the tumor population and cytoplasmic staining. 22C3: Antibody from Dako (diluted 1/50, 30-min incubation at RT) in combination with Dako EnVisionFLEX Target Retrieval Solution (low pH) and the Envision Flex detection system; Excessive background staining and cytoplasmic staining. 28-8: Antibody from Abcam (diluted 1/50, 20-min incubation at 32 °C) in combination with Dako EnVisionFLEX Target Retrieval Solution (low pH) and the Envision Flex detection system; background staining. SP142: RTU antibody from Ventana (16-min incubation at 36 °C) in combination with the Ventana CC1 (48 min) and OptiView DAB IHC Detection Kit; weak staining of epithelial cells. E1L3N: Antibody from Cell Signaling (diluted 1/150, 60-min incubation at RT) in combination with laboratory developed antigen retrieval by TRIS-EDTA and the Vectastain ABC immunoperoxidase staining avidin-biotin complexes; weak demonstration of antigen in the tumor population and cytoplasmic staining. Abbreviations: EQA external quality assessment, FFPE formalin-fixed paraffin embedded, IHC immunohistochemistry, PD-L1 programmed death ligand 1, RT room temperature, RTU ready-to-use, TPS tumor proportion score

    Journal: Virchows Archiv

    Article Title: PD-L1 immunohistochemistry in non-small-cell lung cancer: unraveling differences in staining concordance and interpretation

    doi: 10.1007/s00428-020-02976-5

    Figure Lengend Snippet: Examples of optimal and suboptimal concordance with PD-L1 IHC reference stains for different protocols during the 2018 EQA scheme. Images represent a matching core with a validated consensus TPS of > 50%. The core was part of a tissue micro-array containing four different FFPE cases for staining by routine antibodies and detection systems of the 2018 EQA participants. Protocols are presented as reported by the participating laboratories. Scale bar 1 mm. Optimal concordance (top row, from left to right): SP263: RTU antibody from Ventana (16 min incubation at 36 °C) in combination with the Ventana CC1 (64 min.) and OptiView DAB IHC Detection Kit. 22C3: Antibody from Dako (diluted 1/40, 16-min incubation at 37 °C) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit. 28-8: Antibody from Abcam (diluted 1/100, 32-min incubation at RT) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit. SP142: RTU antibody from Ventana (24-min incubation at 37 °C) in combination with the Ventana CC1 (48 min) and UltraView DAB IHC Detection Kit. E1L3N: Antibody from Cell Signaling (diluted 1/200, 30-min incubation at RT) in combination with Leica Bond Epitope Retrieval 2 (20 min) and bond polymer refine detection system. Suboptimal concordance (bottom row, from left to right): SP263: RTU antibody from Ventana (60-min incubation at 37 °C) in combination with the Ventana CC1 (64 min) and OptiView DAB IHC Detection Kit; weak demonstration of antigen in the tumor population and cytoplasmic staining. 22C3: Antibody from Dako (diluted 1/50, 30-min incubation at RT) in combination with Dako EnVisionFLEX Target Retrieval Solution (low pH) and the Envision Flex detection system; Excessive background staining and cytoplasmic staining. 28-8: Antibody from Abcam (diluted 1/50, 20-min incubation at 32 °C) in combination with Dako EnVisionFLEX Target Retrieval Solution (low pH) and the Envision Flex detection system; background staining. SP142: RTU antibody from Ventana (16-min incubation at 36 °C) in combination with the Ventana CC1 (48 min) and OptiView DAB IHC Detection Kit; weak staining of epithelial cells. E1L3N: Antibody from Cell Signaling (diluted 1/150, 60-min incubation at RT) in combination with laboratory developed antigen retrieval by TRIS-EDTA and the Vectastain ABC immunoperoxidase staining avidin-biotin complexes; weak demonstration of antigen in the tumor population and cytoplasmic staining. Abbreviations: EQA external quality assessment, FFPE formalin-fixed paraffin embedded, IHC immunohistochemistry, PD-L1 programmed death ligand 1, RT room temperature, RTU ready-to-use, TPS tumor proportion score

    Article Snippet: , Homebrew EDTA or TRIS-EDTA (w/wo pressure cooker) , ABC immunoperoxidase staining avidin-biotin complexes (Vectastain ABC Elite; Vector Laboratories) , 1 (0.7%) , 0 (0.0%) , 1 (5.0%) , 0 (0.0%) , 1.0.

    Techniques: Microarray, Staining, Incubation, Immunoperoxidase Staining, Avidin-Biotin Assay, Formalin-fixed Paraffin-Embedded, Immunohistochemistry

    Analysis failures, TPS misclassifications, and ESS for the different PD-L1 IHC protocols used in the EQA schemes

    Journal: Virchows Archiv

    Article Title: PD-L1 immunohistochemistry in non-small-cell lung cancer: unraveling differences in staining concordance and interpretation

    doi: 10.1007/s00428-020-02976-5

    Figure Lengend Snippet: Analysis failures, TPS misclassifications, and ESS for the different PD-L1 IHC protocols used in the EQA schemes

    Article Snippet: , Homebrew EDTA or TRIS-EDTA (w/wo pressure cooker) , ABC immunoperoxidase staining avidin-biotin complexes (Vectastain ABC Elite; Vector Laboratories) , 1 (0.7%) , 0 (0.0%) , 1 (5.0%) , 0 (0.0%) , 1.0.

    Techniques: Immunoperoxidase Staining, Avidin-Biotin Assay, Plasmid Preparation